848 Concise Book of Medical Laboratory Technology: Methods and Interpretations

MUCOLYTIC, DISINFECTANT, SPECIMEN

PRETREATMENT AND BUFFERING SYSTEM

FOR AFB STAINING AND CULTURE

Lyfectol®

(Courtesy: Tulip Group of Companies)

Summary

Infection with Mycobacterium tuberculosis remains a major

public health problem. The epidemic of tuber-culosis and

multidrug resistant tuberculosis reflects the failure of public

health and social programs towards prompt treatment

of infected cases and screening of high-risk population.

Culture, isolation and sensitivity of Mycobacterium

tuberculosis from patient groups using standard methods

remain the gold standard for Mycobacterium tuberculosis

detection and effective and swift treatment worldwide.

Reagent

LYFECTOL is a reagent for laboratory use only. LYFECTOL

is provided as a three component reagent.

a. Reagent A (2% NaOH solution)

b. Reagent B (N- acetyl L-cysteine)

c. Reagent C (Phosphate buffer pH 6.8).

Accessories: Spatula for approximate weighing (12 mg)

and transfer of reagent B.

LYFECTOL is used for decontamination and concentration of specimen containing normal microbial flora

such as sputum as per international recommendation.

Principle

Proper decontamination and concentration of specimen

containing normal microbial flora such as sputum are

crucial in detecting Mycobacterium tuberculosis.

LYFECTOL provides a liquefaction-decontamination

and specimen buffering procedure that maintains the

viability and pathogenicity of Mycobacterium tuberculosis,